![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K231001:Design
Designed by: Daniel R Tarjan Group: iGEM09_Virginia (2009-10-21)
Phytochelatin Synthase, HA-tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507
Illegal NgoMIV site found at 1449 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for E. coli.
Source
Original PCS enzyme: GenBank: AAL66747.1