Composite

Part:BBa_K231001:Design

Designed by: Daniel R Tarjan   Group: iGEM09_Virginia   (2009-10-21)


Phytochelatin Synthase, HA-tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
    Illegal NgoMIV site found at 1449
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E. coli.


Source

Original PCS enzyme: GenBank: AAL66747.1

References